Subscribe to our Newsletter


For Updates on Biomass Analysis Techniques and Biomass Composition

x

  
Message

Email added to list. If you don't receive a confirmation email check your spam folder and add Celignis as a trusted sender.

This website is using cookies
We use cookies to ensure that we give you the best experience on our website. If you continue using the site, we'll assume that you are happy to receive all cookies on this website.
x

  • Facebook
  • Facebook
  • Facebook
  • YouTube
  • Facebook
Extractives (Water-Insoluble, Ethanol Soluble)


The Water-Insoluble but Ethanol-Soluble Extractives content is considered to be the total mass proportion of the biomass that is lost as a result of extraction with 95% ethanol (following a previous extraction with water). It is equivalent to the Exhaustive Extractives content minus the Water-Soluble Extractives content.

Click here for more information on the extractives fraction of biomass.

Click here to place an order for determining Extractives (Water-Insoluble, Ethanol Soluble) .





Analysis Packages for Extractives (Water-Insoluble, Ethanol Soluble)

The Celignis Analysis Package(s) that determine this constituent are listed below:

P6     Full Extractives
Extractives (Ethanol-Soluble), Extractives (Water-Soluble), Extractives (Exhaustive - Water then Ethanol), Extractives (Water-Insoluble, Ethanol Soluble)

Further Details


P10   Sugars, Lignin, Extractives, and Ash
Total Sugars, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Extractives (Ethanol-Soluble), Extractives (Water-Soluble), Extractives (Exhaustive - Water then Ethanol), Extractives (Water-Insoluble, Ethanol Soluble) , Ash, Ash (Acid Insoluble)

Further Details


Analytical Procedure for Extractives (Water-Insoluble, Ethanol Soluble)

Step 1: Removal of Water-Soluble and Ethanol-Soluble Extractives
The steps involved in the removal of Water-Soluble and 95%-Ethanol Soluble Extractives from samples are listed below:

1. The moisture content of the sample is determined, in duplicate.

2. Six 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.

3. The following DIONEX ASE 200 Method is then used on the first 4 cells:

   Pressure:   1500 psi
   Temperature:   100 celcius
   Preheat Time:   0 mins
   Heat Time:   5 mins
   Static Time:   7 mins
   Flush Volume:   150%
   Purge Time:   150 s
   Static Cycles:   3
   Solvent:   Water


4. The Method listed above is then repeated for cells 3-6 with the only difference being that 95%-Ethanol is used as the solvent.

5. A box, of known weight, is taken and the remaining biomass from the first extraction cell transferred to it. This is repeated for the other cells.

6. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).

7. The weight of Water-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the first two cells.

8. The weight of 95%-Ethanol-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the last two cells.

9. The weight of Exhaustive Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for cells 3 and 4.

10. The weight of Water-Insoluble but Ethanol-Soluble Extractives is determined from subtracting the Water-Soluble Extractives content from the Exhaustive Extractives content.

Equipment Used for Extractives (Water-Insoluble, Ethanol Soluble) Analysis

Additional Material

Click here to read more about our methods for analysing extractives.

We can determine the Extractives (Water-Insoluble, Ethanol Soluble) content of biomass, click here to learn more about our various biomass analysis methods.

analysts