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Analysis Package P6:
Full Extractives
flasks

Constituents Determined

For every constituent determined via wet-chemical analysis each sample is analysed in duplicate and we provide the compositional data for each replicate along with the average value and the standard deviation between the duplicates. Our analytical protocols typically provide results with a high level of precision, as detailed here.

Examples of the data reports generated can be viewed on the Celignis Database. Please log on to the guest account using email "test@celignis.com" and password "celignis".

Click here to place an order for determining Full Extractives.



Sample Weight Requirements

Under nornal conditions there is a minimum requirement of 30g of sample for this analysis package with a recommended weight of 60+g of sample.

However, it may be possible for us to undertake the anaysis with lower quantities than the minimum specified above. Please email us at info@celignis.com to find out.

Analytical Procedure for Full Extractives

Step 1: Removal of Water-Soluble and Ethanol-Soluble Extractives
The steps involved in the removal of Water-Soluble and 95%-Ethanol Soluble Extractives from samples are listed below:

1. The moisture content of the sample is determined, in duplicate.

2. Six 11 ml capacity DIONEX ASE (Accelerated Solvent Extraction) cells are filled with recorded weights of the sample.

3. The following DIONEX ASE 200 Method is then used on the first 4 cells:

   Pressure:   1500 psi
   Temperature:   100 celcius
   Preheat Time:   0 mins
   Heat Time:   5 mins
   Static Time:   7 mins
   Flush Volume:   150%
   Purge Time:   150 s
   Static Cycles:   3
   Solvent:   Water


4. The Method listed above is then repeated for cells 3-6 with the only difference being that 95%-Ethanol is used as the solvent.

5. A box, of known weight, is taken and the remaining biomass from the first extraction cell transferred to it. This is repeated for the other cells.

6. After 2 days each box is weighed again and the moisture content of a subsample of the extracted biomass determined (in duplicate).

7. The weight of Water-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the first two cells.

8. The weight of 95%-Ethanol-Soluble Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for the last two cells.

9. The weight of Exhaustive Extractives is determined as the mass loss in the biomass sample, due to extraction in the ASE-200 (corrected for moisture), for cells 3 and 4.

10. The weight of Water-Insoluble but Ethanol-Soluble Extractives is determined from subtracting the Water-Soluble Extractives content from the Exhaustive Extractives content.

Equipment Used for Full Extractives





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