Subscribe to our Newsletter


For Updates on Biomass Analysis Techniques and Biomass Composition

x

  
Message

Email added to list. If you don't receive a confirmation email check your spam folder and add Celignis as a trusted sender.

This website is using cookies
We use cookies to ensure that we give you the best experience on our website. If you continue using the site, we'll assume that you are happy to receive all cookies on this website.
x

  • Facebook
  • Facebook
  • Facebook
  • YouTube
  • Facebook
Analysis Package P13:
Sugars and Oligosaccharides in Solution
flasks

This package is particularly suitable for analysing the liquid output streams from biomass pre-treatment, hydrolysis, and other conversion processes. It can be used to determine the total oligomeric sugar content of a liquid and also the relative proportions that each monosaccharide contributes to this total amount. The package involves a number of stages of analysis.

Firstly, the liquid is analysed using our ion chromatography system for the free monosaccharides and disaccharides in solution, just as in package P21 - Sugars in Solution.

Then we subject the liquid to a mild form of acid hydrolysis involving 4% sulphuric acid and 1 hour in an autoclave at 121 degrees Celcius. These conditions will break apart any oligosaccharides into their constituent monosaccharide units.

The liquid is subsequently filtered and analysed again on our IC system, to determine the amounts of various monosaccharides, after correction for any losses in sugars associated with the hydrolysis process. For each monosaccharide there will now be two values, the concentration prior to hydrolysis and that after hydrolysis.

With the exception of fructose (which degrades to hydroxymethylfurfural during the autoclaving step) all monosaccharides should be present in greater concentration post-hydrolysis. Hence, the proportion of each sugar that is present in the original liquid in the oligomeric form can be calculated by subtracting the pre-hydrolysis concentration from the post-hydrolysis concentration. As we report the sucrose and cellobiose contents separately we correct the calculated oligomeric glucan content using those data.

In our reports for this package we report the pre-hydrolysis, post-hydrolysis and oligomeric concentrations of each sugar.

If you are also interested in finding the amounts of various sugar-degradation products in your liquid then, in addition to this analysis package, we would also recommend analysis package P22 - Organic Acids and Furans which can quantify a number of products, including levulinic acid, furfural, and formic acid).

If you are considering this analysis package to evaluate the outcome of your pre-treatment process, we have outlined a recommended set of analysis packages that will allow you to get detailed information about process yields and efficiencies.

Constituents Determined

For every constituent determined via wet-chemical analysis each sample is analysed in duplicate and we provide the compositional data for each replicate along with the average value and the standard deviation between the duplicates. Our analytical protocols typically provide results with a high level of precision, as detailed here.

Examples of the data reports generated can be viewed on the Celignis Database. Please log on to the guest account using email "test@celignis.com" and password "celignis".

Click here to place an order for determining Sugars and Oligosaccharides in Solution.



Sample Weight Requirements

Under nornal conditions there is a minimum requirement of 12g of sample for this analysis package with a recommended weight of 50+g of sample.

However, it may be possible for us to undertake the anaysis with lower quantities than the minimum specified above. Please email us at info@celignis.com to find out.