Lignocellulose HydrolysisLignocellulosic biomass, primarily comprised of cellulose, hemicellulose, and lignin, is an abundant and renewable resource that holds great promise as a source of biofuels and renewable biobased chemicals and biomaterials. Lignocellulosic biomass can be processed in a number of ways, one is through the hydrolysis of the structural polysaccharides (cellulose and hemicellulose) into their constituent sugars, a reaction commonly facilitated by acid or enzymes, followed by the fermentation of these sugars by yeast or other microorganisms.
Enzymatic HydrolysisIn enzymatic hydrolysis cellulases and hemicellulases play a critical role, working synergistically to cleave the glycosidic linkages in cellulose and hemicellulose, respectively. However, depending on the type of pretreatment process involved, hydrolysis of hemicellulose may not be necessary, since it may have already taken place in the pretreatment leading to the hemicellulose sugars being in the liquid output of the pretreatment with the solid residue mostly containing cellulose (plus lignin, again dependent on the type of pretreatment).
SSF Process and its AdvantagesSimultaneous Saccharification and Fermentation (SSF) is a process for the production of sugar-derived products (such as bioethanol) from lignocellulosic biomass. The process involves the concurrent breakdown (hydrolysis) of cellulose (and hemicellulose, if present) into monomeric sugars (saccharification), and the conversion of these sugars into products via fermentation. This process is distinct from the Separate Hydrolysis and Fermentation (SHF) method, in which saccharification and fermentation occur in separate stages.
Disadvantages of SSF
Fed-Batch SSFFed-batch Simultaneous Saccharification and Fermentation (SSF) is a modification of the traditional SSF process which can, in certain circumstances, improve process efficiencies. In the traditional SSF process, all the components – the lignocellulosic biomass, enzymes, and yeast – are mixed together at the beginning of the process. However, in fed-batch SSF these components are not all added at once. Instead, the lignocellulosic biomass is added in incremental portions or "batches" to the fermenter over the course of the fermentation. By gradually feeding the biomass into the fermenter, it is possible to maintain a more consistent level of sugars throughout the process, which can help to optimize yeast activity and product yields. In addition, this approach can help to alleviate issues related to high solid loading, such as mixing problems and high viscosity.
1. Understanding Your Requirements
2. Detailed Feedstock Analysis
3. Pretreatment (Lab-Scale)
4. SSF Optimisation
5. Product Recovery
6. Valorisation of Remaining Biomass
7. Validation at Higher TRLs
8. Technoeconomic Analysis (TEA)
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PhD (Analytical Chemistry)
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