Processes for the Extraction of Bioactives from Leaves
Background
Bioactive compounds are defined as chemicals, typically found in small amounts in biomass, that have actions in the body that can promote good
health. As a result, many bioactive compounds are considered to be highly valuable in the food, feed, cosmetic, and pharmaceutical industries.
Many bioactives and other high-value plant constituents can be considered to be a part of the biomass extractives, which are defined as extraneous components that may be separated from the insoluble cell wall material by their solubility in water or neutral organic solvents.
Click here to learn more about the ways in which bioprocesses can be developed to efficiently and sustainably extract these extractives from biomass, with particular selectivity towards the bioactives and chemicals of interest.
Get more info...Extraction Processes
Many bioactives and other high-value plant constituents can be considered to be a part of the biomass extractives, which are defined as extraneous components that may be separated from the insoluble cell wall material by their solubility in water or neutral organic solvents.
Click here to learn more about the ways in which bioprocesses can be developed to efficiently and sustainably extract these extractives from biomass, with particular selectivity towards the bioactives and chemicals of interest.
Get more info...Extraction Processes
Bioactives in Herbal Leaf Extracts
Herbal extracts are usually obtained from leaves of the plants.
Some of the well-known herbal leaves are basil, oregano, bay leaf, thyme, tarragon, mint, marjoram, sage,
curry leaf, parsley, rosemary, olive leaves, lime leaves, leaves from Aloaceae or Liliaceae family, and gingko.
Leaves contain valuable bioactive compounds such as tocopherols, carotenoids, flavonoids, sterols, vitamins, tannins, lipids, and minerals which can be used in health, beauty and food industry.
Preparation of herbal leaf extracts requires careful harvesting of leaves, cleaning, grading, sorting and milling. In some cases, additional steps such as aging, steaming, sweating, roasting/frying are required to remove any toxic compounds present in the leaves. Drying of leaves after sorting will stabilise the leaves from bacterial contamination, but may affect the concentration of desired bioactive components.
Leaves contain valuable bioactive compounds such as tocopherols, carotenoids, flavonoids, sterols, vitamins, tannins, lipids, and minerals which can be used in health, beauty and food industry.
Preparation of herbal leaf extracts requires careful harvesting of leaves, cleaning, grading, sorting and milling. In some cases, additional steps such as aging, steaming, sweating, roasting/frying are required to remove any toxic compounds present in the leaves. Drying of leaves after sorting will stabilise the leaves from bacterial contamination, but may affect the concentration of desired bioactive components.
Extraction of bioactive components from leaves can be done by traditional methods such as maceration, infusion, percolation, decoction or
advanced techniques such as enzyme treatments, microwave treatment, ultrasonic extraction, pressurised liquid extraction, and sub-critical or
super critical fluid extraction methods. Advanced techniques reduce the solvent use,
time and energy required for the extraction and increase the yields.
At Celignis we understand your feedstock and your market needs and use advanced techniques to develop optimised economic and environment-friendly extraction processes.
At Celignis we understand your feedstock and your market needs and use advanced techniques to develop optimised economic and environment-friendly extraction processes.
Further Info on Classes of Bioactives in Herbal Leaf Extracts
Tocopherols are a type of organic compound known collectively as vitamin E.
They are fat-soluble antioxidant that have the ability to neutralize harmful free radicals in fat-soluble environments in the body.
There are four different types of tocopherols: alpha, beta, gamma, and delta.
Examples of some tocopherols found in herbal leaf extracts are listed below:
Get more info...Tocophenols
Examples of some tocopherols found in herbal leaf extracts are listed below:
- Tea Leaves - Green tea and black tea leaves contain alpha-tocopherol and gamma-tocopherol.
- Herbs and Spices - Certain herbs and spices, like oregano and paprika, are also rich in tocopherols. They contain mainly gamma-tocopherol, although alpha-tocopherol can be present as well.
- Green Leafy Vegetables - These are known to contain alpha-tocopherol, the most biologically active form of Vitamin E. Spinach and kale, for example, are two leafy greens that are a good source of this tocopherol.
- Cannabis Leaves - Some research has found that cannabis leaves contain alpha-tocopherol and gamma-tocopherol.
Get more info...Tocophenols
Carotenoids are a group of plant pigments responsible for bright red, yellow, and orange hues seen in many
fruits and vegetables. They can also have beneficial
properties for human health.
Examples of some carotenoids found in herbal leaf extracts are listed below:
Get more info...Carotenoids
Examples of some carotenoids found in herbal leaf extracts are listed below:
- Beta-Carotene - found in various green leafy vegetables and herbs.
- Lutein and Zeaxanthin - These carotenoids are typically found together and are especially abundant in dark green leafy vegetables like spinach and kale.
- Lycopene - While lycopene is more commonly associated with red fruits and vegetables, particularly tomatoes, some herbs also contain this antioxidant carotenoid.
- Astaxanthin - This carotenoid is most commonly found in algae and seafood like salmon and shrimp, but it can also be found in some herbal extracts.
- Beta-Cryptoxanthin - Another pro-vitamin A carotenoid like beta-carotene, beta-cryptoxanthin can also be found in certain herbal leaf extracts.
Get more info...Carotenoids
Flavonoids are a diverse group of plant compounds that belong to the larger class of phytonutrients known as polyphenols.
Examples of some flavonoids found in herbal leaf extracts are listed below:
Get more info...Flavonoids
Examples of some flavonoids found in herbal leaf extracts are listed below:
- Quercetin - It is one of the most abundant flavonoids found in plants and is widely distributed in herbal leaves. It is known for its antioxidant and anti-inflammatory properties. Quercetin-rich herbs include onions, kale, broccoli, and green tea.
- Kaempferol - Found in various herbs and leafy vegetables like kale, spinach, and tea leaves. It also has antioxidant and anti-inflammatory properties and has been associated with potential benefits for heart health and cancer prevention.
- Apigenin - This is commonly found in chamomile, parsley, and celery leaves. Apigenin has been studied for its potential to inhibit cancer cell growth, promote relaxation, and support sleep quality.
- Luteolin - This flavonoid is present in certain herbal leaves such as thyme, peppermint, and celery. It has been investigated for its potential neuroprotective effects and immune-modulating properties.
- Rutin - A flavonoid glycoside that can be found in herbs like buckwheat, as well as citrus fruits and other plant sources. It is known for its antioxidant properties and has been studied for its potential benefits in strengthening blood vessels and reducing the risk of cardiovascular diseases.
Get more info...Flavonoids
Sterols are a type of lipid,
structurally similar to cholesterol, that play essential roles in various biological processes.
Examples of some sterols found in herbal leaf extracts are listed below:
Get more info...Sterols
Examples of some sterols found in herbal leaf extracts are listed below:
- Beta-Sitosterol - This is one of the most prevalent plant sterols found in herbal extracts. It is structurally similar to cholesterol and can be found in various plant sources, including herbs like saw palmetto, pygeum, and stinging nettle.
- Campesterol - This sterol has been investigated for its potential cholesterol-lowering effects and may contribute to the cardiovascular health benefits associated with plant sterol consumption.
- Stigmasterol - Found in various herbal extracts, including those derived from soybeans, fenugreek, and black cohosh. It has been studied for its potential anti-inflammatory and hormone-modulating effects.
- Delta-7-Sitosterol - A plant sterol found in certain herbal extracts, including those derived from saw palmetto and pumpkin seeds. It is often associated with potential benefits for prostate health.
- Other Plant Sterols - Brassicasterol, avenasterol, and stigmastanol, are examples of other sterols that can be found in herbal leaf extracts.
Get more info...Sterols
Tannins are a group of compounds belonging to a larger class of substances known as polyphenols, which are a type of antioxidant.
Some points regarding tannins in herbal leaf extracts are provided below:
Get more info...Tannins
Some points regarding tannins in herbal leaf extracts are provided below:
- Plant Types - Herbs like witch hazel, sage, rosemary, and yarrow are known for their high tannin contents.
- Astringency - Tannins in herbal leaf extracts are responsible for the astringent properties of many of these herbs. Astringency is the drying, tightening, and sometimes puckering sensation that you experience when you consume certain foods or drinks. For example, it's the tannins that give black tea, red wine, and certain fruits like unripe apples or persimmons their characteristic mouthfeel.
- Hydrolyzable Tannins - These are found in some herbs like witch hazel (Hamamelis virginiana). Two types of phenolic acids commonly found in hydrolyzable tannins are gallic acid and ellagic acid.
Get more info...Tannins
Analysis of Bioactives in Herbal Leaf Extracts
We have the necessary equipment and expertise to determine the amount and distribution of bioactives in
the extracts obtained from a wide variety of biomass samples.
Feel free to click on the links above to see more information on our methods of analysis for the different classes of bioactives or get in touch with us to request further information and a quotation.
Request a QuoteBioactives Analysis
Feel free to click on the links above to see more information on our methods of analysis for the different classes of bioactives or get in touch with us to request further information and a quotation.
Request a QuoteBioactives Analysis
Bioactives According to Other Plant Fractions
Bark is rich in bioactives like phenolics, tannins, flavonoids, terpenoids, sterols, and glycosides.
Bark extracts are used for anti-microbial, anti-inflammatory, and wound-healing properties.
Get more info...Bark Extracts
Get more info...Bark Extracts
Roots are rich in terpenoids, alkaloids and flavonoids. Flavonoids are known to have signalling function in root-rhizosphere and
constitute a large part of root exudate.
Get more info...Root Extracts
Get more info...Root Extracts
Floral extracts are rich in pigments (betalains, carotenoids and anthocyanins). Flowers are also rich source of other bioactive components
such as resins and sterols.
Get more info...Flower Extracts
Get more info...Flower Extracts
Fruits and vegetables are rich sources of vitamins, phenolics, carotenoids, anthocyanins, and tocopherols. These bioactives
are known for their anti-oxidant and anti-microbial properties.
Get more info...Fruit & Veg Extracts
Get more info...Fruit & Veg Extracts
Seeds contain many secondary metabolites (e.g. alkaloids, saponins, phenolics, sterols). The bioactives composition varies
depending on the location in the seed (seed coat, endosperm and embryo).
Get more info...Seed Extracts
Get more info...Seed Extracts
Identification of High-Value Chemicals
For identification, we firstly get a crude extract from the feedstock, obtained via various approaches, including pressurised liquid extraction. This extract is then profiled using our top-range QTOF-LC/MS system (Agilent iFunnel 6550), which can identify constituents to the femtogram-level, and the spectra and chromatograms reviewed by Sajna, our Bioanalysis Developer. If necessary, we can collect the relevant fractions from the LC system and confirm the identification using a number of different chemical and spectroscopic techniques. We then determine which constituents warrant extraction.
Develop an Optimised Extraction Protocol
Based on the identified chemical(s) of interest, we can then work on optimising a targeted extraction method. This method considers not only the yield of the target compound(s) but also the chemical and energy costs of the process and the implications for the downstream processing and valorisation of the solid residue. We consider a range of different extraction technologies, solvents, and process conditions.Typically, we first optimise the extraction at lab-scale conditions and then validate the chosen set of conditions at an nehanced scale (i.e. a higher technology readiness level (TRL)).
Separation and Purification of Target Chemical(s)
We can work on a process for the separation and purification of the targeted high value extractive compounds, while considering commercial viability and environmental aspects such as solvent and energy consumption. Based on the particular bioactive compound of interest and the composition of the liquid extract, we can employ a variety of different techniques for separation and purification.Application Testing of Extract or Separated Components
We can test the extract, or the separated compounds of interest, for a variety of different applications. The tests are custom-designed based on the extracted compound. For low molecular weight biomolecules (non food and feed), bioactivity tests, anti-microbial tests, UV absorbance properties, surface activities, and emulsion forming abilities can be tested. For the food and feed related extract fractions, such as proteins and carbohydrate polymers, nutritional properties, anti-nutritional compounds, emulsification, gelation, foaming (together with bioactive properties, in vitro digestibility etc.) are tested.Technoeconomic Analysis of the Process
The Celignis team, including Oscar our chief TEA expert, can undertake a detailed technoeconomic analysis of the developed process. We apply accurate and realistic costing models to determine the CAPEX and OPEX of simulated and pilot scale processes which are then used to determine key economic indicators such as IRR, NPV and payback periods.Our sensitivity analyses can assess the effect of variable costs and revenues, a particularly important aspect to consider given that the sale prices for some bioactive compounds can vary significantly according to their purity and market conditions.
Our preferred approach is to include TEA studies at each stage of the development of the extraction bioprocess, so that the process can be optimised in a commercially-relevant way, followed by a more detailed TEA after the process has been optimised and tested at higher TRL levels.
Click here to read more about the technoeconomic analysis (TEA) services offered by Celignis.
Bioprocess Biomass Extraction Projects - Case Studies
Betulin - UNRAVEL Project
Celignis worked extensively on biomass extraction and the purification of bioactive chemicals in the CBE/BBI project UNRAVEL where the extractives of 25 feedstocks were profiled using our QTOF-LC/MS system. We identified betulin in birch bark as the most attractive compound and subsequently worked on developing an optimised extraction protocol and an isolation/purification process scheme that offered several advantages, in terms of sustainability and safety, over the current art. Click here for a news article on this work.
SteamBioAfrica Project
We are also using employing our compositional analysis and purification expertise in the Horizon Europe project SteamBioAfrica where we evaluate and process the liquid condensate obtained from the steam torrefaction process and consider market applications for its constituents, fractions, and derivatives.Celignis is testing the separation of acids, phenolics, and aldehydes for their potential to be used as high value bioactive compounds and biopesticides.
Bioactives Profiling of Tropical Trees
Celignis undertook detailed analysis of a wide variety of tropical hardwood trees for a client. This involved characterisation of different anatomical fractions (e.g. stem wood, bark, foliage). There was a particular focus on the composition of the extractives of these feedstocks. We used our QTOF-LC/MS system to profile the diverse and complex array of bioactive compounds present in the samples. We then evaluated the identified compounds and selected key chemicals that could be of high potential value for sale in different markets. We then undertook a review of these compounds, considering their potential value in various markets, the processes that could be required for their separation and purification, and whether other compounds could also be obtained as part of the extraction/separation process. The final output of the project was a list of top feedstocks and chemicals for future bioprocess development.
Our Bioprocess Development Services can work on the evaluation and optimisation of a particular node in the biomass
processing technology or can involve the development of a bespoke vertically-integrated technology for your chosen feedstock
and/or product. Click here to see how our Bioprocess Development Services work and how
we devise and undertake a project.
With regards to the extraction of bioactives from biomass, the Celignis Bioprocess team members with the most experience in undertaking such projects are listed below. Feel free to contact them to discuss potential projects.
With regards to the extraction of bioactives from biomass, the Celignis Bioprocess team members with the most experience in undertaking such projects are listed below. Feel free to contact them to discuss potential projects.
Lalitha Gottumukkala
Founder of Celignis Bioprocess, CIO of Celignis
PhD
Has a deep understanding of all biological and chemical aspects of bioproceses. Has developed Celignis into a renowned provider of bioprocess development services to a global network of clients.
Dan Hayes
Celignis CEO And Founder
PhD (Analytical Chemistry)
Dreamer and achiever. Took Celignis from a concept in a research project to being the bioeconomy's premier provider of analytical and bioprocessing expertise.
Sajna KV
Bioanalysis Developer
PhD
Our Biomass Detective! Designs, tests, optimizes and validates robust analytical methods for our bioprocess development projects. Such bespoke analysis is key to developing an optimised bioprocess.
See our pitches for the 2024 topics.
Special Offer
Mar 30th 2024
CBE-JU 2024 Call Opens - See our Pitches for Proposals
Click here to view our pitches for involvement in proposals for the 2024 research topics of the Circular Bioeconomy Europe Joint Undertaking (CBE-JU).
Analysis Packages
P8 : Lignin Content
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
P19 : Deluxe Lignocellulose Package
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
P15 : Uronic Acids
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
P121 : Evaluation of Biomass for Enzymatic Digestibility
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
P122 : Evaluation of Pre-Treatment on Enzymatic Digestibility
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
P123 : Composition of Residue from Enzymatic Hydrolysis
As P9 but on the solid residue after enzymatic hydrolysis.
As P9 but on the solid residue after enzymatic hydrolysis.
P124 : Fermentation Inhibitors in Enzymatic Hydrolysate
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
P125 : Cellulase Saccharification Efficiency
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
P126 : Xylanase Saccharification Efficiency
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
P127 : Amylase Saccharification Efficiency
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
P12 : Sugars in Solvent Extract
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
P22 : Organic Acids and Furans
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
P24 : Dimers and Trimers from Hemicellulose Hydrolysis
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
P29 : Oligomers from Starch Hydrolysis
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
P15 : Uronic Acids
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
P75 : Seaweed Phytohormones
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
P76 : Seaweed Vitamins (Fat-Soluble)
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
P77 : Seaweed Vitamins (Water-Soluble)
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
P71 : Seaweed Carbohydrates
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
P72 : Seaweed Amino Acids
Alanine, Arginine, Aspartic Acid, Cystine, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, Valine,
Alanine, Arginine, Aspartic Acid, Cystine, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, Valine,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P73 : Seaweed Lipids as Fatty Acids
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
P74 : Pigments in Seaweed
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
P75 : Seaweed Phytohormones
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
P76 : Seaweed Vitamins (Fat-Soluble)
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
P77 : Seaweed Vitamins (Water-Soluble)
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
P150 : Plant Pigments
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
P81 : Biomethane Potential - 14 Days
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
P93 : Feedstock Chemical and Biological Analysis
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Phosphorus, Potassium, Ammonia, Carbon, Hydrogen, Nitrogen, Sulphur,
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD), Biological Oxygen Demand (BOD), Phosphorus, Potassium, Ammonia, Carbon, Hydrogen, Nitrogen, Sulphur,
P95 : Residual Biogas Potential - 14 Days
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
P222 : Volatile Fatty Acids (VFAs) Speciation
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
P61 : Sugars in Bio-Oil Water Extract
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
P63 : Semi-Volatile Oxygenated Components in Bio-Oil
31 constituents including Phenol, Furfural, Syringol, and Vanillin
31 constituents including Phenol, Furfural, Syringol, and Vanillin
P360 : Specific Surface Area (5-Point BET)
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
P364 : Pore-Size Distribution
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
P366 : Pore Size Distribution Deluxe
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
P34 : Calorific Value and Elements
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P42 : Ash Melting Behaviour (Reducing Conditions)
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
P393 : Biochar Thermal Properties Deluxe
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
P394 : Biochar Thermal Properties Ultimate
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P384 : Biochar Polycyclic Aromatic Hydrocarbons (PAH)
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
P388 : Biochar Plant Growth Trials
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
P397 : Biochar Soil Amendment Ultimate
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
P399 : Biochar Complete Evaluation Package
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
P34 : Calorific Value and Elements
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P40 : Combustion Package
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
P41 : Ash Melting Behaviour (Oxidising Conditions)
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
News
Jan 24th 2024
€1.5m Funding Success in CBE-JU
Celignis is a Partner in 3 Successful Proposals for EU Funding
We are pleased to announce that three of the proposals involving Celignis, submitted to the CBE-JU programme for funding collaborative biomass research in Europe, were successful. These projects will provide an additional funding of €1.5m to Celignis and build on our achievements in other CBE and EU projects. In particular, the projects are all at enhanced TRLs (6/7) and will use our existing Celignis Bioprocess infrastructure and will also fund further development of our bioprocessing capacities and the Bioprocess Development Services we offer our clients.
Details on the funded projects are provided below:
BIONEER - This project was funded under CBE-JU topic IA-06 and focuses on the TRL 6/7 production of biobased platform chemicals. Celignis's activities in the project focus on scaling up the work undertaken in our ongoing
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Jan 23rd 2024
Celignis to Exhibit and Present at Major Biochar Event
The 2024 North American Biochar Conference will take place in Sacramento, California, on Feb 12-15
On Feb 12-15 we'll be exhibiting at the 2024 North American Biochar Conference, taking place at the SAFE Credit Union Convention Centre in Sacramento, California.
We're looking forward to interacting with the 1000+ expected attendees, outlining our extensive range of analytical and application testing services for biochar.
Celignis CIO Lalitha Gottumukkala will also be a member of the expert panel focused on developing improved laboratory methods for biochar characterisation.
Click here to register for the event.
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Jan 22nd 2024
Celignis Attending BIC Event on Feb 8
This Networking Event Will Involve Discussions on Collaborations for Proposals to the 2024 CBE-JU Topics
The Circular Bioeconomy Europe Joint Undertaking (CBE-JU) is an organisation that funds biomass research in Europe at various Technology Readiness Levels (TRLs). Since 2016 Celignis has been an active participant in a number of projects funded by the CBE-JU.
The Biobased Industries Consortium (BIC) is the steering committee that helps to steer the focus of research for the CBE-JU programme. In 2023 Celignis joined the BIC as a Full Industry Member and participated in several proposals submitted for different research topics in the CBE-JU's 2023 Work Programme.
On Feb 8th Celignis's Dan Hayes, Lalitha Gottumukkala, and Oscar Bedzo will be attending a BIC networking event in Brussels where we will discuss potential collaborations in the research programme topics recently announced for 2024.
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Jan 19th 2024
We're Hiring - Business Administration & Client Relationship Manager
This position will involve working closely with senior management, fostering existing and new client relationships.
Situated in Limerick, Ireland, Celignis currently operates at two centres, Celignis Analytical and Celignis Bioprocess, actively engaging in a variety of private and public bioeconomy projects. As we continue to expand, we're looking to strengthen our team of 14 with a Business Administration and Client Relationship Manager who can bring a blend of enthusiasm and expertise.
This position will involve working closely with senior management, fostering existing and new client relationships, and ensuring successful delivery of our services, playing a key role in our ongoing growth and success.
Click here for more details about the position.
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Apr 30th 2023
Celignis to Sponsor and Present at Major Biochar Event
The event takes place on May 3rd at Carrick-on-Shannon
We are pleased to announce that, on May 3rd, Celignis will be presenting and exhibiting at the National Biochar and Carbon Products Conference 2023, which is taking place in Carrick-on-Shannon in County Leitrm, Ireland.
This conference is being organised under the auspices of the Interreg Northwest Europe-funded THREE C Project, entitled 'Creating and sustaining Charcoal value chains to promote a Circular Carbon economy in NWE Europe'.
The conference will highlight both Irish stakeholders who are currently working in the biochar and carbon products sector, but also partners from the THREE C project (covering Netherlands, Luxembourg, Germany, Belgium, France and Wales, as well as Ireland) who have interesting stories and products to share.
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