• Steam Preatmeant
    Including Steam Explosion
    Bioprocess Development


Lignocellulosic Biomass

Lignocellulosic biomass is defined as a plant, or plant-derived, material that is mostly composed of cellulose, hemicellulose, and lignin. Lignocellulosic feedstocks are highly abundant, covering many biomass types including grasses, wood, energy crops (e.g. Miscanthus and coppices), agricultural residues (e.g. straws and corn stover), and municipal wastes.

Lignocellulosic feedstocks are highly abundant and can often be sourced sustainably, at low cost, without leading to land-use conflicts. As a result, there is currently great interest in obtaining chemicals, fuels, and biomaterials from such biomass.

Biomass Hydrolysis

A major pathway by which many lignocellulosic feedstocks are processed is known as hydrolysis, where sugars are released from the lignocellulosic polysaccharides (i.e. cellulose and hemicellulose).

However, the hydrolysis of lignocellulosic polysaccharides is not easy and is influenced by the complex inter-associations between hemicellulose and cellulose and between these polysaccharides and lignin in the lignocellulosic matrix. In particular, the crystalline nature of much cellulose and the existence of a physical barrier of lignin surrounding the cellulose fibres are said to be major contributors to the recalcitrance of cellulose.

The mechanism of hydrolysis is further complicated by the fact that different process intensities are required for the hydrolysis of cellulose versus hemicellulose. The more intense conditions required for cellulose hydrolysis may degrade the sugars hydrolysed from hemicellulose (to products such as furfural and formic acid).

For this reason, most hydrolysis technologies employ pre-treatment processes that aim to break apart the matrix (and in particular the associations between lignin and cellulose), reduce cellulose crystallinty, and hydrolyse hemicelluloses, hence separating the hydrolysate from cellulose which can then undergo more severe/targeted treatment.

Get more info...Biomass Hydrolysis

Need for Pretreatment

For this reason, most hydrolysis technologies employ pre-treatment processes that aim to break apart the matrix (and in particular the associations between lignin and cellulose), reduce cellulose crystallinty, and hydrolyse hemicelluloses, hence separating the hydrolysate from cellulose which can then undergo more severe/targeted treatment.

Get more info...Pretreatment Homepage

Steam Pretreatment (and Steam-Explosion Pretreatment) of Biomass

Steam pretreatment technologies, including those covering steam explosion pretreatment, have been an active area of research for the several decades. The process, also known as steam autohydrolysis, targets the deconstruction of the complex lignocellulosic matrix.

In steam pretreatment the lignocellulosic biomass is exposed to high-pressure steam with the temperature and pressure maintained for a specified duration, depending on the biomass type and the desired outcome. The action of steam at high pressure disrupts the lignocellulosic structure, partially hydrolyzing the hemicellulose component into simpler sugars and breaking the lignin seal that surrounds the cellulose fibres. This makes the cellulose more accessible for subsequent enzymatic hydrolysis, where cellulose is broken down into glucose.

The resulting liquid stream after steam pretreatment, often called hydrolysate, contains dissolved hemicellulose-derived sugars and some soluble lignin and other degradation products. The remaining solid fraction contains cellulose, residual hemicellulose, and lignin.

The difference between steam pretreatment and steam-explosion pretreatment is that the latter involves a rapid decompression step that physically disrupts the biomass structure.

Steam pretreatments can take place uncatalysed or catalysed with dilute sulphuric acid. Additionally, ammonia fibre explosion (AFEX pretreatment) substitutes liquid ammonia for steam.

Effects of Different Steam Pretreatment Process Conditions

  • Temperature and Pressure - These two factors are directly related as per the principles of thermodynamics. Higher temperatures and pressures generally lead to more effective disruption of the lignocellulosic structure, leading to greater cellulose accessibility. However, excessively high temperatures may lead to the degradation of some sugars, particularly the more labile hemicellulose-derived sugars, into undesirable and potentially inhibitory compounds like furfural and hydroxymethylfurfural (HMF). It can also lead to the formation of more lignin-derived phenolic compounds, which can also be inhibitory to subsequent enzymatic and microbial processes.
  • Duration - The length of the steam treatment also plays a significant role. Longer exposure times at the set temperature and pressure may result in more thorough hemicellulose hydrolysis and cellulose exposure. However, similar to higher temperatures, longer treatment times can also lead to higher levels of sugar degradation and the production of inhibitory compounds.
  • Explosive Decompression - This is a unique step in the steam explosion process. The severity of the explosion (i.e. the extent of the pressure drop) can impact the degree of biomass disruption and the resulting composition.

Feedstock Considerations in Steam Pretreatment

  • Extractives - The content and composition of the extractives is important in steam pretreatment for several reasons. For example, some extractives might create a protective layer around the biomass, reducing the effectiveness of the steam pretreatment process by acting as a barrier to the steam. Additionally, some extractives can degrade under the high temperatures used in steam pretreatment and so form compounds that are inhibitory to subsequent enzymatic hydrolysis or fermentation processes. These degradation products can also cause issues with equipment fouling and corrosion.
  • Hemicellulose Type - The type of hemicellulose can influence how readily it is hydrolyzed during steam pretreatment. For instance, xylans tend to be more readily hydrolyzed than mannans. A major reason why this is the case is that xylans contain acetyl groups. During steam pretreatment, these acetyl groups can be cleaved off, releasing acetic acid. This can acidify the pretreatment solution, which can help to catalyze the hydrolysis of hemicellulose. However, too much acetic acid can lead to inhibition of subsequent enzymatic hydrolysis and fermentation.
  • Lignin Type - The composition of lignin, particularly the ratio of syringyl (S), guaiacyl (G), and p-hydroxyphenyl (H) units, can influence the outcomes of steam pretreatment. For instance, biomass with a higher S/G ratio (like hardwoods and some grasses) tends to be easier to pretreat than biomass with a lower S/G ratio (like softwoods).

Advantages of Steam Pretreatment

  • Efficient Hemicellulose Hydrolysis - Steam pretreatment is effective in hydrolyzing the hemicellulosic fraction of biomass into soluble sugars.
  • Enhanced Enzymatic Digestibility - By breaking down the complex structure of lignocellulosic biomass and partially removing or redistributing lignin, steam pretreatment enhances the accessibility of cellulose to enzymatic hydrolysis.
  • No Chemical Requirement - Steam pretreatment is often referred to as "autohydrolysis". The lack of cehmical use (compared to some other types of pretreatments) can lead to cost savings and a more environmentally friendly process.
  • Flexibility - Steam pretreatment can be applied to a variety of types of biomass, making it a flexible option for biorefineries dealing with different feedstocks.
  • Scalability - The process is relatively easy to scale-up for industrial applications, which is an important consideration for commercial biorefinery operations.
  • Potential for Energy Recovery - The high-pressure steam used in the process can potentially be recovered and reused, improving the overall energy efficiency of the process.

Disadvantages of Steam Pretreatment

  • Formation of Inhibitory Compounds - One of the main challenges with steam pretreatment is the formation of inhibitory compounds, such as furfural and hydroxymethylfurfural (HMF), which are degradation products of sugars, as well as various lignin-derived phenolic compounds. These compounds can inhibit subsequent enzymatic hydrolysis and fermentation processes.
  • Incomplete Lignin Removal - Steam pretreatment may not effectively remove or redistribute all the lignin present in the biomass. The remaining lignin can limit the accessibility of cellulose to enzymes in subsequent hydrolysis steps, reducing the overall efficiency of sugar release.
  • Energy Requirement - The process requires substantial amounts of energy to generate the high-pressure steam.
  • Equipment Requirements - The high pressures and temperatures used in steam pretreatment require robust and often expensive specialized equipment. Also, operation at these conditions might lead to quicker wear and tear of the equipment, increasing maintenance costs.
  • Optimization for Different Biomass Types - While steam pretreatment is generally applicable to many different types of biomass, each type might require different pretreatment conditions for optimal results. Therefore, when changing between different feedstocks, the process may need to be re-optimized.

Steam Pretreatment Bioprocess Development at Celignis

Whether you have an exisiting steam pretreatment process that you think can be improved, or if you want to evaluate steam pretreatment for your feedstock(s), we are here to help.

Our initial step would involve learning from you what your targets are for the pretreatment and what the subsequent stages of the process, and final products, should be.

Then we would undergo a detailed compositional analysis of the feedstock in order to gain an understanding of its suitability for steam pretreatment and to consider appropriate process conditions.

We would then work on a design of experiments (DoE) for lab-scale steam pretreatments in order to explore the effects of different process conditions.

There would be detailed analysis of the liquid and solid outputs of these pretreatments, comparing these results with the targeted aims of the pretreatment and downstream valorisation.

If subsequent enzymatic hydrolysis of the solid residue is the planned next step in the process then we can undertaken lab-scale enzymatic hydrolysis experiments on the residues from this DoE. We can also use the cellulose-derived sugars as a substrate for fermentation, combining the approaches if required (i.e. simultaneous saccharification and fermentation).

If there are also plans for downstream valorisation of the liquid fraction we can also assess the suitability of various hydrolysates from the DoE for such processes.

Following a review of the results from the DoE we can then work on iterative scale-ups of the process. The outputs of these scaled-up pretreatments can also be tested for their downstream valorisations. Any deviations from the outputs at lower TRLs can be assessed and remedying approaches undertaken.

The commercial viability of pretreatment is particularly important and should be considered throughout the project. We usually recommend to our clients that we undertake interative techno-economic analyses (TEA) at various stages of the bioprocess development, so that the experimental and scale-up work are framed in a commercially-relevant context.

Get more info...How our Bioprocess Projects Work

Other Types of Biomass Pretreament

Mechanical Pretreatment

Mechanical pretreatments of biomass usually focus on a reduction in the particle size of the feedstock, allowing for more surface-area availability in downstream processes.

Get more info...Mechanical Pretreatment

Hydrothermal Pretreatment

Hydrothermal pretreatment, also know as Liquid Hot Water (LHW) pretreatment, does not involve the use of any chemicals but operates at elevated temperatures and pressures

Get more info...Hydrothermal Pretreatment

Acid Pretreatment

This pretreatment involves treating the biomass with a dilute solution of a strong acid at elevated temperatures. A primary target is the hydrolysis of hemicellulose into monomeric sugars.

Get more info...Acid Pretreatment

Alkali Pretreatment

Alkali pretreatment uses chemicals (e.g. sodium hydroxide, potassium hydroxide, calcium hydroxide) to disrupt the complex structure of lignocellulosic biomass with lignin removal a primary target.

Get more info...Acid Pretreatment

Organosolv Pretreatment

Organosolv pretreatment fractionates biomass into its three major components (cellulose, hemicellulose, and lignin), with obtaining a lignin of a high-purity and quality being a particular target.

Get more info...Organosolv Pretreatment

Other Pretreatments

This page covers some other pretreatments: ionic liquids pretreatment, hydrogen-peroxide pretreatment, ultrasonic pretreatment, microwave pretreatment, fungal pretreatment, and bacterial pretreatment.

Get more info...Other Pretreatments

Pretreatment Homepage

Click here to read more about the important factors to consider when designing a biomass pretreatment process and to read about how we undertake our pretreatment development projects with our clients.

Get more info...Pretreatment Homepage


How our Bioprocess Development Services Work

Our Bioprocess Development Services can work on the evaluation and optimisation of a particular node in the biomass processing technology or can involve the development of a bespoke vertically-integrated technology for your chosen feedstock and/or product. Click here to see how our Bioprocess Development Services work and how we devise and undertake a project.

With regards to the pretreatment of biomass, the Celignis Bioprocess team members with the most experience in undertaking such projects are listed below. Feel free to contact them to discuss potential projects.

Lalitha Gottumukkala

Founder of Celignis Bioprocess, CIO of Celignis


Has a deep understanding of all biological and chemical aspects of bioproceses. Has developed Celignis into a renowned provider of bioprocess development services to a global network of clients.

Oscar Bedzo

Bioprocess Project Manager & Technoeconomic Analysis Lead


A dynamic, purpose-driven chemical engineer with expertise in bioprocess development, process design, simulation and techno-economic analysis over several years in the bioeconomy sector.

Dan Hayes

Celignis CEO And Founder

PhD (Analytical Chemistry)

Dreamer and achiever. Took Celignis from a concept in a research project to being the bioeconomy's premier provider of analytical and bioprocessing expertise.