Mechanical Pretreatment of Biomass
Background
Lignocellulosic Biomass
Lignocellulosic biomass is defined as a plant, or plant-derived, material that is mostly composed of cellulose, hemicellulose, and lignin. Lignocellulosic feedstocks are highly abundant, covering many biomass types including grasses, wood, energy crops (e.g. Miscanthus and coppices), agricultural residues (e.g. straws and corn stover), and municipal wastes.Lignocellulosic feedstocks are highly abundant and can often be sourced sustainably, at low cost, without leading to land-use conflicts. As a result, there is currently great interest in obtaining chemicals, fuels, and biomaterials from such biomass.
However, the hydrolysis of lignocellulosic polysaccharides is not easy and is influenced by the complex inter-associations between hemicellulose and cellulose and between these polysaccharides and lignin in the lignocellulosic matrix. In particular, the crystalline nature of much cellulose and the existence of a physical barrier of lignin surrounding the cellulose fibres are said to be major contributors to the recalcitrance of cellulose.
The mechanism of hydrolysis is further complicated by the fact that different process intensities are required for the hydrolysis of cellulose versus hemicellulose. The more intense conditions required for cellulose hydrolysis may degrade the sugars hydrolysed from hemicellulose (to products such as furfural and formic acid).
For this reason, most hydrolysis technologies employ pre-treatment processes that aim to break apart the matrix (and in particular the associations between lignin and cellulose), reduce cellulose crystallinty, and hydrolyse hemicelluloses, hence separating the hydrolysate from cellulose which can then undergo more severe/targeted treatment.
Get more info...Biomass Hydrolysis
Need for Pretreatment
For this reason, most hydrolysis technologies employ pre-treatment processes that aim to break apart the matrix (and in particular the associations between lignin and cellulose), reduce cellulose crystallinty, and hydrolyse hemicelluloses, hence separating the hydrolysate from cellulose which can then undergo more severe/targeted treatment.Get more info...Pretreatment Homepage
Mechanical Pretreatment of Biomass
It is generally the case that the smaller the particle size, the higher the efficiency of the process. However, excessive reduction in particle size may cause process issues (e.g. viscosity, high water holding, filter clogging etc.).
Depending on the pretreatment conditions, and on the particle-size reduction tools used in the process, the pretreatment process may also cause the biomass to shear and reduce the degree of polymerization, which in turn increases the available accessible area to chemicals, enzymes and microbes.
Other Types of Biomass Pretreament
Get more info...Steam Pretreatment
Get more info...Hydrothermal Pretreatment
Get more info...Acid Pretreatment
Get more info...Acid Pretreatment
Get more info...Organosolv Pretreatment
Get more info...Other Pretreatments
Get more info...Pretreatment Homepage
With regards to the pretreatment of biomass, the Celignis Bioprocess team members with the most experience in undertaking such projects are listed below. Feel free to contact them to discuss potential projects.
Lalitha Gottumukkala
Founder of Celignis Bioprocess, CIO of Celignis
PhD
Has a deep understanding of all biological and chemical aspects of bioproceses. Has developed Celignis into a renowned provider of bioprocess development services to a global network of clients.
Oscar Bedzo
Bioprocess Project Manager & Technoeconomic Analysis Lead
PhD
A dynamic, purpose-driven chemical engineer with expertise in bioprocess development, process design, simulation and techno-economic analysis over several years in the bioeconomy sector.
Dan Hayes
Celignis CEO And Founder
PhD (Analytical Chemistry)
Dreamer and achiever. Took Celignis from a concept in a research project to being the bioeconomy's premier provider of analytical and bioprocessing expertise.
See our pitches for the 2024 topics.
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P9 but on the solid residue after enzymatic hydrolysis.
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
Alanine, Arginine, Aspartic Acid, Asparagine, Cystine, Glutamic Acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD),
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
31 constituents including Phenol, Furfural, Syringol, and Vanillin
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
Celignis is a Partner in 3 Successful Proposals for EU Funding
We are pleased to announce that three of the proposals involving Celignis, submitted to the CBE-JU programme for funding collaborative biomass research in Europe, were successful. These projects will provide an additional funding of €1.5m to Celignis and build on our achievements in other CBE and EU projects. In particular, the projects are all at enhanced TRLs (6/7) and will use our existing Celignis Bioprocess infrastructure and will also fund further development of our bioprocessing capacities and the Bioprocess Development Services we offer our clients.
Details on the funded projects are provided below:
BIONEER - This project was funded under CBE-JU topic IA-06 and focuses on the TRL 6/7 production of biobased platform chemicals. Celignis's activities in the project focus on scaling up the work undertaken in our ongoing
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The 2024 North American Biochar Conference will take place in Sacramento, California, on Feb 12-15
On Feb 12-15 we'll be exhibiting at the 2024 North American Biochar Conference, taking place at the SAFE Credit Union Convention Centre in Sacramento, California.
We're looking forward to interacting with the 1000+ expected attendees, outlining our extensive range of analytical and application testing services for biochar.
Celignis CIO Lalitha Gottumukkala will also be a member of the expert panel focused on developing improved laboratory methods for biochar characterisation.
Click here to register for the event.
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This Networking Event Will Involve Discussions on Collaborations for Proposals to the 2024 CBE-JU Topics
The Circular Bioeconomy Europe Joint Undertaking (CBE-JU) is an organisation that funds biomass research in Europe at various Technology Readiness Levels (TRLs). Since 2016 Celignis has been an active participant in a number of projects funded by the CBE-JU.
The Biobased Industries Consortium (BIC) is the steering committee that helps to steer the focus of research for the CBE-JU programme. In 2023 Celignis joined the BIC as a Full Industry Member and participated in several proposals submitted for different research topics in the CBE-JU's 2023 Work Programme.
On Feb 8th Celignis's Dan Hayes, Lalitha Gottumukkala, and Oscar Bedzo will be attending a BIC networking event in Brussels where we will discuss potential collaborations in the research programme topics recently announced for 2024.
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This position will involve working closely with senior management, fostering existing and new client relationships.
Situated in Limerick, Ireland, Celignis currently operates at two centres, Celignis Analytical and Celignis Bioprocess, actively engaging in a variety of private and public bioeconomy projects. As we continue to expand, we're looking to strengthen our team of 14 with a Business Administration and Client Relationship Manager who can bring a blend of enthusiasm and expertise.
This position will involve working closely with senior management, fostering existing and new client relationships, and ensuring successful delivery of our services, playing a key role in our ongoing growth and success.
Click here for more details about the position.
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The event takes place on May 3rd at Carrick-on-Shannon
We are pleased to announce that, on May 3rd, Celignis will be presenting and exhibiting at the National Biochar and Carbon Products Conference 2023, which is taking place in Carrick-on-Shannon in County Leitrm, Ireland.
This conference is being organised under the auspices of the Interreg Northwest Europe-funded THREE C Project, entitled 'Creating and sustaining Charcoal value chains to promote a Circular Carbon economy in NWE Europe'.
The conference will highlight both Irish stakeholders who are currently working in the biochar and carbon products sector, but also partners from the THREE C project (covering Netherlands, Luxembourg, Germany, Belgium, France and Wales, as well as Ireland) who have interesting stories and products to share.
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