Production of Polyhydroxy Alkanoates (PHAs) Through Fermentation
Background to Polyhydroxy Alkanoates (PHAs) Fermentation
PHAs are biodegradable, organic, non-toxic polyesters that are produced by certain bacteria and plants. PHA granules accumulated in the microbial cell are called carbonosomes.
They serve as storage reservoirs and hence the fermentation needs feast and famine regimes in a sequential system or in a single system. Simple fed-batch systems due to their dilution effect on the media make
it complicated to achieve targeted PHA yields, resulting in the subsequent downstream processing being less economically viable.
An alternative is to use repeated fed-batch and fed-batch with cell recycling to allow accumulation of biomass and PHAs in the biomass. There is growing interest in using mixed cultures on mixed waste streams to reduce the costs associated with aseptic mono-culture fermentation.
An alternative is to use repeated fed-batch and fed-batch with cell recycling to allow accumulation of biomass and PHAs in the biomass. There is growing interest in using mixed cultures on mixed waste streams to reduce the costs associated with aseptic mono-culture fermentation.
Though this approach can be optimised by following the same feast and famine regime for high PHA yields, there is an issue with copolymer blends that will be produced with varying microstructure, physical,
and chemical properties causing unpredictable product quality. This can be solved by adding a pre-biomass conversion step to the PHA fermentation process and enriching the PHA accumulating bacteria by dynamic
feast and famine cultivation. Chemostats are reported to be more suitable for mixed microbial cultivation as enriched bacteria can be retained unlike batch and fed-batch fermentation where fresh bacterial inoculum is
added at every start-phase which is many times more frequent than chemo-stat systems. Unconventional substrates like gaseous substrates are being tried with unconventional PHA producers
e.g. Cyanobacteria to explore the potential of converting syngas and biogas to PHAs with or without additional carbon source in the growth media.
How Celignis Can Help
PHA is one of the most complicated fermentation processes, but the possibility to use mixed microbial cultures and avoiding sterilisation costs
makes it an interesting process to produce bioplastics. Also, PHA blends are becoming more and more popular to increase the tensile strength and flexibility
of the polymer which is possible by using mixed culture substrates.
At Celignis, we have expertise and experience in enrichment of desired microorganisms, fed-batch and continuous fermentations with cell-recycling. We can design and develop the most suitable process for your feedstock by using mixed or mono-culture fermentations. We can also develop cost-efficient downstream processing steps for efficient PHA extraction by using non-toxic and environmentally friendly techniques.
Our team of experts will innovate with you for you.
Get more info...Get in Touch
At Celignis, we have expertise and experience in enrichment of desired microorganisms, fed-batch and continuous fermentations with cell-recycling. We can design and develop the most suitable process for your feedstock by using mixed or mono-culture fermentations. We can also develop cost-efficient downstream processing steps for efficient PHA extraction by using non-toxic and environmentally friendly techniques.
Our team of experts will innovate with you for you.
Get more info...Get in Touch
Lactic Acid Fermentation
Lactic acid bacteria are very sensitive and require complex nutrient media compared to other bacillus species that can produce lactic acid.
Hence, industries are constantly looking for fungi and bacillus strains that have low nutrient requirements and can tolerate acidic pH.
At Celignis we have expertise and experience in screening lactic acid bacteria for the selection of substrate- and product-tolerant strains. We can also develop: fed-batch strategies to achieve high cell mass, and in situ product recovery techniques to separate lactic acid from the fermentation broth. We will work with you and develop bespoke lactic acid fermentation methods for your feedstock or industrial waste streams.
Get more info...Lactic Acid Fermentation
At Celignis we have expertise and experience in screening lactic acid bacteria for the selection of substrate- and product-tolerant strains. We can also develop: fed-batch strategies to achieve high cell mass, and in situ product recovery techniques to separate lactic acid from the fermentation broth. We will work with you and develop bespoke lactic acid fermentation methods for your feedstock or industrial waste streams.
Get more info...Lactic Acid Fermentation
Propionic Acid Fermentation
Propionic acid can be produced from a variety of substrates such as glucose, ethanol, lactose, glycerol, and pectin. So, several industrial streams
will be suitable to produce propionic acid, if the bacteria are adapted to the inhibitors present in the waste streams and fermentation is optimised to
achieve high cell densities and high product concentration.
We can perform anaerobic fermentations and develop fermentation strategies to achieve high cell mass and in-situ product recovery techniques. We can screen your feedstock for propionic acid production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery processes.
Get more info...Propionic Acid Fermentation We can perform anaerobic fermentations and develop fermentation strategies to achieve high cell mass and in-situ product recovery techniques. We can screen your feedstock for propionic acid production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery processes.
Butyric Acid Fermentation
Butyric acid is biologically produced by Clostridium species and like other acids (acetic acid, lactic acid, propionic acid),
it is toxic to the bacteria after a certain concentration. Hence, the product titres are generally low which makes downstream expensive.
In order to reduce these costs, in situ removal of butyric acid can be developed. In situ removal strategies are not yet industrially applied for butyric acid, but
it is a key area where progress has to be made to make the process economically sustainable.
At Celignis, we have strong expertise in Clostridial fermentation. We can isolate and or adapt the strains that are suitable for your feedstock and can develop fermentation strategies to reduce substrate and product inhibition. We will innovate with you for you.
Get more info...Butyric Acid Fermentation
At Celignis, we have strong expertise in Clostridial fermentation. We can isolate and or adapt the strains that are suitable for your feedstock and can develop fermentation strategies to reduce substrate and product inhibition. We will innovate with you for you.
Get more info...Butyric Acid Fermentation
Butanol Fermentation
Butanol fermentation is also one of the difficult fermentation pathways due to substrate and product inhibition. However, this can be
avoided by fed-batch fermentation and in-situ stripping of butanol. Also, reducing the feedstock and enzyme costs will make the process more
economically viable. Through using industrial waste streams (negative costs), enzymatic cocktails tailored for the feedstock (allowing low-enzyme dosages), and
with high sugar yields, the right choice of microbial strain, and an effective in-situ removal technology, it is possible to develop an economically-viable butanol process.
At Celignis, we have considerable expertise in Clostridial fermentation and especially butanol fermentation. Our Chief Innovation Officer Dr Lalitha Gottumukkala has extensively worked in this area and has isolated novel strains and developed novel methods for non-acetogenic butanol fermentation as part of her PhD.
Get more info...Butanol Fermentation
At Celignis, we have considerable expertise in Clostridial fermentation and especially butanol fermentation. Our Chief Innovation Officer Dr Lalitha Gottumukkala has extensively worked in this area and has isolated novel strains and developed novel methods for non-acetogenic butanol fermentation as part of her PhD.
Get more info...Butanol Fermentation
1,3-Propanediol Fermentation
Natural microbes that produce 1,3-Propanediol are Klebsiella, Clostridia, Citrobacter, Enterobacter
and Lactobacilli. They all use glycerol as a carbon source and produce 1,3 PDO through 3-hydroxypropionaldehyde route
using glycerol dehydratase enzymes and 1,3-propanediol oxidoreductase enzymes.
At Celignis, we have expertise and experience in performing anaerobic fermentations and developing fermentation strategies to achieve high cell mass and in situ product recovery techniques. We can screen your feedstock for 1,3-Propanediol production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery process.
Get more info...1,3-PDO Fermentation
At Celignis, we have expertise and experience in performing anaerobic fermentations and developing fermentation strategies to achieve high cell mass and in situ product recovery techniques. We can screen your feedstock for 1,3-Propanediol production, adapt the strain to any inhibitors present in the feed, and develop bespoke fermentation and product recovery process.
Get more info...1,3-PDO Fermentation
Yeast and Fungal Fermentation
Yeast fermentation is one of the oldest fermentations and is used in everyday life to produce a variety of commodity products including bread, beer, wine,
cheese, and soy sauce. A few decades ago, yeast gained popularity as an industrial strain for biorefinery and biofuel applications.
At Celignis, we can use yeast and fungi for the production of: bioethanol, glycerol, single cell oils (SCOs), and emulsifiers, among other products.
Get more info...Yeast and Fungal Fermentation At Celignis, we can use yeast and fungi for the production of: bioethanol, glycerol, single cell oils (SCOs), and emulsifiers, among other products.
Microalgal Fermentation
Algal cultivation is complicated and requires optimisation to achieve high biomass yields. Algal biomass production depends on nutrient uptake and
other environmental conditions such as temperature, pH, salt concentration etc. It is important to select the strain based on the type of
production (open ponds, photobioreactors), feedstock and application. We have particular expertise in the evaluation and optimisation of algae thorugh
our Chief Innovation Officer, Lalitha, who is currently undertaking a Marie-Curie funded project at Celignis on this topic.
Get more info...Microalgae Fermentation 
Contact Us For Further Details
We are available to answer any questions you may have on how to get high value chemicals and biofuels from biomass through fermentation processes.
Just get in touch with us by sending us an email info@celignis.com, giving us a call at (+353) 61 371 725, or through
our contact form.
Get more info...Get in Touch
Get more info...Get in Touch
See our pitches for the 2024 topics.
Analysis Packages
P8 : Lignin Content
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
Lignin (Klason), Lignin (Acid Soluble), Acid Insoluble Residue, Ash (Acid Insoluble),
P19 : Deluxe Lignocellulose Package
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
As P10 plus protein-corrected lignin, water-soluble sugars, uronic acids, acetyl content and starch.
P15 : Uronic Acids
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
P121 : Evaluation of Biomass for Enzymatic Digestibility
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Xylose in Enzyme Hydrolysate, Arabinose in Enzyme Hydrolysate, Mannose in Enzyme Hydrolysate, Galactose in Enzyme Hydrolysate, Rhamnose in Enzyme Hydrolysate, Cellobiose in Enzyme Hydrolysate, Enzymatic Hydrolysis Kinetics, Cellulose Conversion Yield, Xylan Conversion Yield, Combined Sugar Yield, Cellulose Conversion Rate, Xylan Conversion Rate,
P122 : Evaluation of Pre-Treatment on Enzymatic Digestibility
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
As P121 plus comparisons with data from the non-pretreated original sample, including: Increase in Cellulose Accessibility after Pre-Treatment, Percent Increase in Cellulose Conversion Efficiency, Percent Increase in Cellulose Conversion Rate.
P123 : Composition of Residue from Enzymatic Hydrolysis
As P9 but on the solid residue after enzymatic hydrolysis.
As P9 but on the solid residue after enzymatic hydrolysis.
P124 : Fermentation Inhibitors in Enzymatic Hydrolysate
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
Formic Acid, Acetic Acid, Levulinic Acid, Furfural, Hydroxymethylfurfural,
P125 : Cellulase Saccharification Efficiency
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Cellulose Conversion Yield, Cellulose Conversion Rate
P126 : Xylanase Saccharification Efficiency
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
Includes all hydrolysate sugars and kinetics in P121 and: Xylan Conversion Yield, Xylan Conversion Rate
P127 : Amylase Saccharification Efficiency
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
Total Sugars in Enzyme Hydrolysate, Glucose in Enzyme Hydrolysate, Maltose in Enzyme Hydrolysate, a-Amylase Hydrolysis Kinetics, Glucoamylase Hydrolysis Kinetics,
P12 : Sugars in Solvent Extract
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
Glucose, Xylose, Fructose, Sucrose, Mannose, Arabinose, Galactose, Rhamnose, Xylitol, Sorbitol, Trehalose, Mannitol, Arabinitol, Glycerol, Raffinose,
P22 : Organic Acids and Furans
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
Levulinic Acid, Formic Acid, Hydroxymethylfurfural, Furfural, Acetic Acid, gamma-Valerolactone,
P24 : Dimers and Trimers from Hemicellulose Hydrolysis
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
Xylobiose, Xylotriose, Arabinobiose, Arabinotriose,
P29 : Oligomers from Starch Hydrolysis
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
Maltose, Maltotriose, Maltotetraose, Maltopentaose, Maltohexaose, Maltoheptaose, Maltooctaose,
P15 : Uronic Acids
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, 4-O-Methyl-D-Glucuronic Acid, Iduronic Acid,
P75 : Seaweed Phytohormones
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
P76 : Seaweed Vitamins (Fat-Soluble)
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
P77 : Seaweed Vitamins (Water-Soluble)
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
P71 : Seaweed Carbohydrates
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
Fucose, Mannitol, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Total Sugars, Glucuronic Acid, Galacturonic Acid, Mannuronic Acid, Guluronic Acid, Iduronic Acid,
P72 : Seaweed Amino Acids
Alanine, Arginine, Aspartic Acid, Asparagine, Cystine, Glutamic Acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine,
Alanine, Arginine, Aspartic Acid, Asparagine, Cystine, Glutamic Acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine, Threonine, Tryptophan, Tyrosine, Valine,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P73 : Seaweed Lipids as Fatty Acids
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
Arachidic Acid, Behenic Acid, Decanoic Acid, Erucic Acid, Lauric Acid, Linoleic Acid, Linolenic Acid, Myristic Acid, Caprylic Acid, Oleic Acid, Palmitic Acid, Palmitoleic Acid, Stearic Acid, Lignoceric Acid,
P74 : Pigments in Seaweed
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
Fucoxanthin, Astaxanthin, Chlorophyll-c, Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Antheraxanthin, Violaxanthin,
P75 : Seaweed Phytohormones
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
Gibberellic Acid, Indole-3-acetic acid, Indole-2-acetic acid, Indole-3-propionic acid, Indole-3-butyric acid, 6-Benzylaminopurine, Kinetin riboside, Abscisic acid, Salicylic acid,
P76 : Seaweed Vitamins (Fat-Soluble)
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
beta-Carotene, Ergocalciferol (Vitamin D2), Alpha-tocopherol (vitamin E), Phylloquinone (Vitamin K1),
P77 : Seaweed Vitamins (Water-Soluble)
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
Thiamine (Vitamin B1), Riboflavin (Vitamin B2), Niacin (Vitamin B3), Niacinamide (vitamin B3), Pantothenic Acid (Vitamin B5), Pyridoxine (Vitamin B6), Folate (Vitamin B9), Cobalamin (Vitamin B12), Ascorbic Acid (Vitamin C),
P150 : Plant Pigments
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
Chlorophyll-a, Chlorophyll-b, Lutein, beta-Carotene, Neoxanthin, Astaxanthin, Zeaxanthin, Antheraxanthin, Violaxanthin,
P81 : Biomethane Potential - 14 Days
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Biomethane Potential (BMP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
P93 : Feedstock Chemical and Biological Analysis
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD),
Total Solids, Volatile Solids, pH, Chemical Oxygen Demand (COD),
P95 : Residual Biogas Potential - 14 Days
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
Residual Biogas Potential (RBP), Total Biogas Volume, Total Solids, Volatile Solids, pH, Biogas Methane Content, Biogas Carbon Dioxide Content, Biogas Oxygen Content, Biogas Hydrogen Sulphide Content, Biogas Ammonia Content,
P222 : Volatile Fatty Acids (VFAs) Speciation
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
Acetic Acid, Lactic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, Valeric Acid, Isovaleric Acid,
P61 : Sugars in Bio-Oil Water Extract
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
Levoglucosan, Cellobiosan, Mannosan, Galactosan, Glucose, Xylose, Mannose, Arabinose, Galactose, Rhamnose, Fucose, Sucrose, Cellobiose, Total Sugars,
P63 : Semi-Volatile Oxygenated Components in Bio-Oil
31 constituents including Phenol, Furfural, Syringol, and Vanillin
31 constituents including Phenol, Furfural, Syringol, and Vanillin
P360 : Specific Surface Area (5-Point BET)
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (5 Point Using Nitrogen),
P364 : Pore-Size Distribution
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (20 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
P366 : Pore Size Distribution Deluxe
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
Specific Surface Area (Nitrogen Gas Adsorption), BET Isotherm (40 Point Using Nitrogen), Pore Volume (Using Nitrogen), Pore Size Distribution (Using Nitrogen), Average Pore Width (Using Nitrogen),
P34 : Calorific Value and Elements
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P42 : Ash Melting Behaviour (Reducing Conditions)
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
P393 : Biochar Thermal Properties Deluxe
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
Moisture, Ash Content (815C), Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Chlorine, Volatile Matter, Fixed Carbon, Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium, Gross Calorific Value, Net Calorific Value, Ash Shrinkage Starting Temperature (Reducing), Ash Deformation Temperature (Reducing), Ash Hemisphere Temperature (Reducing), Ash Flow Temperature (Reducing),
P394 : Biochar Thermal Properties Ultimate
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
As P393 plus inorganic carbon, organic carbon, TGA (under nitrogen and air), and inherent moisture
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P384 : Biochar Polycyclic Aromatic Hydrocarbons (PAH)
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
Acenaphthene, Acenaphthylene, Anthracene, Benz[a]anthracene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[ghi]perylene, Benzo[a]pyrene, Chrysene, Dibenz[a,h]anthracene, Fluoranthene, Fluorene, Indeno[1,2,3-cd]pyrene, 1-Methylnaphthalene, 2-Methylnaphthalene, Naphthalene, Phenanthrene, Pyrene,
P388 : Biochar Plant Growth Trials
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
Time to Germination, Mean Shoot Length (Week 1), Mean Shoot Length (Week 2), Mean Shoot Length (Week 3), Mean Shoot Length (Week 4), Shoot Weight (Week 4), Mean Root Length (Week 4), Root Weight (Week 4),
P397 : Biochar Soil Amendment Ultimate
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
As Deluxe package plus P383, SEM Imaging (P387) and Plant Growth Trials (P388)
P399 : Biochar Complete Evaluation Package
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
Includes everything from P391 (Physical Properties Ultimate), P394 (Thermal Properties Ultimate), and P397 (Soil Amendment Ultimate)
P34 : Calorific Value and Elements
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
Gross Calorific Value, Net Calorific Value, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen,
P36 : Major Elements
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
Aluminium, Calcium, Iron, Magnesium, Phosphorus, Potassium, Silicon, Sodium, Titanium,
P37 : Minor Elements
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
Antimony, Arsenic, Cadmium, Chromium, Cobalt, Copper, Lead, Manganese, Mercury, Molybdenum, Nickel, Vanadium, Zinc,
P40 : Combustion Package
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
Volatile Matter, Fixed Carbon, Moisture, Ash, Carbon, Hydrogen, Nitrogen, Sulphur, Oxygen, Gross Calorific Value, Net Calorific Value, Chlorine,
P41 : Ash Melting Behaviour (Oxidising Conditions)
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
Ash Shrinkage Starting Temperature (Oxidising), Ash Deformation Temperature (Oxidising), Ash Hemisphere Temperature (Oxidising), Ash Flow Temperature (Oxidising),
News
Oct 15th 2025
MANUREFINERY 12-Month Meeting in Naples
Celignis will be presenting their outputs from the first 12-months of the project
Celignis's Oscar and Fernando are today attending the 12-month review meeting of our CBE-JU research project MANUREFINERY. The event is taking place at the facility of project partner ENCO in Naples, Italy.
MANUREFINERY, entitled "Smart modular mobile biorefining of manure to zero-waste maximising resource recovery for feed and fertiliser bioingredients in rural areas" is one of the 7 ongoing CBE-JU projects that Celignis is currently involved in. Click here to read more about these projects.
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Jul 3rd 2025
Celignis Hosts EnXylaScope Meeting
Final Review Meeting for the Project
Today we welcomed our partners from the EnXylaScope project to Limerick for the final in-person meeting of the project. The event is being held at the Limerick Strand Hotel and will include a tour of Celignis's Bioprocess and Analytical facilities.
EnXylaScope has been a hugely successful project, leading to multiple innovations in enzyme discovery and biomass processing, with Celignis making impactful developments in technologies for xylan extraction and modification. The project's success has led to a number of follow-on projects (BIONEER and PROMOFER) that involve demonstration of these technologies at higher TRL levels.
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Jun 11th 2025
Lalitha Presents our EnXylaScope Work at EUBCE 2025
Event takes place in Valencia Spain
Lalitha is today presenting the outputs of our EnXylaScope project at the 34th European Biomass Conference & Exhibition (EUBCE) which is being held in Valencia, Spain.
The presentation, entitled "EnXylaScope: Unleashing xylan's potential with enzymes for a scope of consumer products", will provide a wide-ranging overview of the project which is now in its 4th year.
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May 23rd 2025
Celignis Tops 50,000 Samples!
Analytical Milestone Achieved
Today we registered sample number 50,000 on our internal LIMS system. Over the years we have analysed every kind of biomass sample imagineable and we look forward to achieving further milestones in the future as we continue to grow and expand our operations.
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Feb 3rd 2025
Dileep Joins the Celignis Team
Will play a key role as our "Mechanical Engineer for Scaled-up Bioprocesses"
Today the Celignis family welcomed Dileep Raveendran-Nair to the fold. Dileep is a Mechanical Engineer with over 11 years of hands-on experience in biofuel, biochar, and bioproduct process engineering. His career has focused on the operation, maintenance, and optimization of large-scale bioprocess and bioenergy systems, integrating engineering precision with biotechnological innovation to advance sustainable industrial application.
At Celignis he will play a key-role ("Mechanical Engineer for Scaled-up Bioprocesses") in the development of our bioprocessing capabilities as we increase the Technology Readiness Levels (TRL) of our bioprocessing facility to TRL7 and cubic-metre reactors.
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